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Event Date: 
Monday, April 10, 2017 - 3:30pm to 5:00pm

Dr. Lise Arleth, Structural Biophysics, X-ray and Neutron Science, Niels Bohr Institute, University of Copenhagen, Denmark

TITLE: Using Phospholipid Nanodiscs for Small-angle X-ray and Neutron Scattering Based Structural Studies of Membrane Proteins

Small-Angle Neutron Scattering (SANS) and Small-Angle X-ray Scattering (SAXS) have been combined with different approaches for reconstituting membrane proteins and modeling based data analysis to enable the investigation of the structure of a general membrane protein as well as its surrounding lipid membrane environment.  Results obtained for the structure, localization and orientation of three structurally very different membrane protein types, Bacteriorhodopsin, Aquaporin and Cytochrome P450 will be presented. It is shown that the small-angle scattering based approach holds perspectives for investigating both the flexibility and dynamics of membrane proteins in a native-like lipid environment. Results, limitations and perspectives of the developed approach will be discussed.

Lise Arleth received her PhD in Physics in 2002 from Risø National Laboratory and The Royal Danish Veterinary and Agricultural University in Denmark under the supervision of Prof Jan Skov Pedersen. She held a Post-Doctoral fellowship at Lujan Neutron Scattering Center at Los Alamos National Laboratory, New Mexico, from 2002 to 2003. Since returning to Denmark in 2004 she has held academic positions at first Risø National Laboratory, then the Royal Veterinary and Agricultural University and since 2007 at University of Copenhagen. In 2015 she was appointed professor in Experimental Biophysics at the Niels Bohr Institute of Physics at University of Copenhagen. Throughout her career, Lise Arleth has used solution small-angle scattering (X-rays and neutrons, i.e. SAXS and SANS) as her main technique for investigating various self-assembled and colloidal samples. Presently she has special focus on developing approaches for investigating membrane proteins under solution conditions. Almost all experiments are carried out at international large scale facilities (X-ray Synchrotrons and Neutron scattering facilities) in highly interdisciplinary and international scientific environments. Lise Arleth is strongly engaged in the European Spallation Source (ESS) and the Max-IV Synchrotron projects.

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